Virus-induced gene-silencing in wheat spikes and grains and its application in functional analysis of HMW-GS-encoding genes.

ABSTRACT:

Background

The Barley stripe mosaic virus (BSMV)-based vector has been developed and used for gene silencing in barley and wheat seedlings to assess gene functions in pathogen- or insect-resistance, but conditions for gene silencing in spikes and grains have not been evaluated. In this study, we explored the feasibility of using BSMV for gene silencing in wheat spikes or grains.

Results

Apparent photobleaching on the spikes infected with BSMV:PDS at heading stage was observed after 13 days post inoculation (dpi), and persisted until 30 dpi, while the spikes inoculated with BSMV:00 remained green during the same period. Grains of BSMV:PDS infected spikes also exhibited photobleaching. Molecular analysis indicated that photobleached spikes or grains resulted from the reduction

SUBMITTER: Ma M 

PROVIDER: S-EPMC3462119 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature