Progesterone-dependent regulation of endometrial cannabinoid receptor type 1 (CB1-R) expression is disrupted in women with endometriosis and in isolated stromal cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).

ABSTRACT:

Objective

To examine the differentiation-related expression of cannabinoid receptor type 1 (CB1-R) messenger RNA (mRNA) and protein in endometrial tissue obtained from women with and without endometriosis and to determine the impact of acute 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure on CB1-R gene expression in isolated endometrial stromal cells.

Design

Laboratory-based study.

Setting

University-affiliated medical center.

Patient(s)

Women with and without endometriosis undergoing volunteer endometrial biopsies after informed consent.

Intervention(s)

None.

Main outcome measure(s)

Analysis of in vivo CB1-R mRNA and protein expression in human endometrial tissues and mRNA expression in isolated stromal cells after exposure to TCDD or a proge

SUBMITTER: Resuehr D 

PROVIDER: S-EPMC3462249 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature