High-throughput screening for bioactive molecules using primary cell culture of transgenic zebrafish embryos.

ABSTRACT: Transgenic zebrafish embryos expressing tissue-specific green fluorescent protein (GFP) can provide an unlimited supply of primary embryonic cells. Agents that promote the differentiation of these cells may be beneficial for therapeutics. We report a high-throughput approach for screening small molecules that regulate cell differentiation using lineage-specific GFP transgenic zebrafish embryonic cells. After validating several known regulators of the differentiation of endothelial and other cell types, we performed a screen for proangiogenic molecules using undifferentiated primary cells from flk1-GFP transgenic zebrafish embryos. Cells were grown in 384-well plates with 12,128 individual small molecules, and GFP expression was analyzed by means of an automated imaging system, which allowe

SUBMITTER: Huang H 

PROVIDER: S-EPMC3462279 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature