Maternal 3'UTRs: from egg to onset of zygotic transcription in Atlantic cod.

ABSTRACT:

Background

Zygotic transcription in fish embryos initiates around the time of gastrulation, and all prior development is initiated and controlled by maternally derived messenger RNAs. Atlantic cod egg and embryo viability is variable, and it is hypothesized that the early development depends upon the feature of these maternal RNAs. Both the length and the presence of specific motifs in the 3'UTR of maternal RNAs are believed to regulate expression and stability of the maternal transcripts. Therefore, the aim of this study was to characterize the overall composition and 3'UTR structure of the most common maternal RNAs found in cod eggs and pre-zygotic embryos.

Results

22229 Sanger-sequences were obtained from 3'-end sequenced cDNA libraries prepared from oocyte, 1-2 cell, bla

SUBMITTER: Kleppe L 

PROVIDER: S-EPMC3462720 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature