VtaA8 and VtaA9 from Haemophilus parasuis delay phagocytosis by alveolar macrophages.

ABSTRACT: Haemophilus parasuis, a member of the family Pasteurellaceae, is a common inhabitant of the upper respiratory tract of healthy pigs and the etiological agent of Glässer's disease. As other virulent Pasteurellaceae, H. parasuis can prevent phagocytosis, but the bacterial factors involved in this virulence mechanism are not known. In order to identify genes involved in phagocytosis resistance, we constructed a genomic library of the highly virulent reference strain Nagasaki and clones were selected by increased survival after incubation with porcine alveolar macrophages (PAM). Two clones containing two virulent-associated trimeric autotransporter (VtaA) genes, vtaA8 and vtaA9, respectively, were selected by this method. A reduction in the interaction of the two clones with the macrophages wa

SUBMITTER: Costa-Hurtado M 

PROVIDER: S-EPMC3462726 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature