Validation of macromolecular flexibility in solution by small-angle X-ray scattering (SAXS).

ABSTRACT: The dynamics of macromolecular conformations are critical to the action of cellular networks. Solution X-ray scattering studies, in combination with macromolecular X-ray crystallography (MX) and nuclear magnetic resonance (NMR), strive to determine complete and accurate states of macromolecules, providing novel insights describing allosteric mechanisms, supramolecular complexes, and dynamic molecular machines. This review addresses theoretical and practical concepts, concerns, and considerations for using these techniques in conjunction with computational methods to productively combine solution-scattering data with high-resolution structures. I discuss the principal means of direct identification of macromolecular flexibility from SAXS data followed by critical concerns about the methods

SUBMITTER: Hammel M 

PROVIDER: S-EPMC3462898 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature