Annexin A5-conjugated polymeric micelles for dual SPECT and optical detection of apoptosis.

ABSTRACT:

Unlabelled

Imaging of apoptosis can allow noninvasive assessment of disease states and response to therapeutic intervention for a variety of diseases. The purpose of this study was to develop and evaluate a multimodal nanoplatform for the detection of apoptosis.

Methods

To modulate the pharmacokinetics of annexin A5, a 36-kDa protein that binds specifically with phosphatidylserine, annexin A5 was conjugated to polyethylene glycol-coated, core-cross-linked polymeric micelles (CCPMs) dually labeled with near-infrared fluorescence fluorophores and a radioisotope ((111)In). To evaluate the specificity of the binding of annexin A5-CCPM to apoptotic cells, both fluorescence microscopy and cell-binding studies were performed in vitro. Pharmacokinetics, biodistribution, dual nuclear

SUBMITTER: Zhang R 

PROVIDER: S-EPMC3463236 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature