Antibodies covalently immobilized on actin filaments for fast myosin driven analyte transport.

ABSTRACT: Biosensors would benefit from further miniaturization, increased detection rate and independence from external pumps and other bulky equipment. Whereas transportation systems built around molecular motors and cytoskeletal filaments hold significant promise in the latter regard, recent proof-of-principle devices based on the microtubule-kinesin motor system have not matched the speed of existing methods. An attractive solution to overcome this limitation would be the use of myosin driven propulsion of actin filaments which offers motility one order of magnitude faster than the kinesin-microtubule system. Here, we realized a necessary requirement for the use of the actomyosin system in biosensing devices, namely covalent attachment of antibodies to actin filaments using heterobifunctional cr

SUBMITTER: Kumar S 

PROVIDER: S-EPMC3463588 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature