Stabilization of functional recombinant cannabinoid receptor CB(2) in detergent micelles and lipid bilayers.

ABSTRACT: Elucidation of the molecular mechanisms of activation of G protein-coupled receptors (GPCRs) is among the most challenging tasks for modern membrane biology. For studies by high resolution analytical methods, these integral membrane receptors have to be expressed in large quantities, solubilized from cell membranes and purified in detergent micelles, which may result in a severe destabilization and a loss of function. Here, we report insights into differential effects of detergents, lipids and cannabinoid ligands on stability of the recombinant cannabinoid receptor CB(2), and provide guidelines for preparation and handling of the fully functional receptor suitable for a wide array of downstream applications. While we previously described the expression in Escherichia coli, purification and

SUBMITTER: Vukoti K 

PROVIDER: S-EPMC3463599 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature