Routine cryopreservation of spermatozoa is safe--evidence from the DNA methylation pattern of nine spermatozoa genes.

ABSTRACT:

Purpose

Assess short- and mid-term impact of cryopreservation on DNA methylation status of different genes in spermatozoa.

Methods

Semen samples from 10 healthy normozoospermic men were collected at the Department of Clinical Andrology of the Centre of Reproductive Medicine and Andrology (Muenster, Germany). Each was divided into four equal aliquots: 1) untreated, 2) diluted in cryoprotectant, 3) short term (2 days) cryopreserved and 4) mid term (4 weeks) cryopreserved. Samples were "swim-up" purified prior to analysis. DNA fragmentation was measured using comet assay and Flow cytometric evaluation with Acridine Orange (FCEAO). The degree of methylation of nine genes was determined by bisulfite pyrosequencing of genomic DNA.

Result(s)

Analysis of three maternally impr

SUBMITTER: Klaver R 

PROVIDER: S-EPMC3463656 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature