Possible mechanism of polyspermy block in human oocytes observed by time-lapse cinematography.

ABSTRACT:

Purpose

To analyze the fertilization process related to polyspermy block in human oocytes using an in vitro culturing system for time-lapse cinematography.

Methods

We had 122 oocytes donated for this study from couples that provided informed consent. We recorded human oocytes at 2,000 to 2,800 frames every 10 s during the fertilization process and thereafter every 2 min using a new in vitro culture system originally developed by the authors for time-lapse cinematography. We displayed 30 frames per second for analysis of the polyspermy block during fertilization.

Results

Three oocytes showed the leading and following sperm within the zona pellucida in the same microscopic field. The dynamic images obtained during the fertilization process using this new system revealed

SUBMITTER: Mio Y 

PROVIDER: S-EPMC3463667 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature