Large-scale quantitative glycoproteomics analysis of site-specific glycosylation occupancy.

ABSTRACT: Disease-associated aberrant glycosylation may be protein specific and glycosylation site specific. Quantitative assessment of glycosylation changes at a site-specific molecular level may represent one of the initial steps for systematically revealing the glycosylation abnormalities associated with a disease or biological state. Comparative quantitative profiling of glycoproteome to provide accurate quantification of site-specific glycosylation occupancy has been a challenging task, requiring a concerted approach drawing from a variety of techniques. In this report, we present a quantitative glycoproteomics method that allows global scale identification and comparative quantification of glycosylation site occupancy using mass spectrometry. We further demonstrated this approach by quantitati

SUBMITTER: Pan S 

PROVIDER: S-EPMC3463725 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature