Microfluidic chips with reversed-phase monoliths for solid phase extraction and on-chip labeling.

ABSTRACT: The integration of sample preparation methods into microfluidic devices provides automation necessary for achieving complete micro total analysis systems. We have developed a technique that combines on-chip sample enrichment with fluorescence labeling and purification. Polymer monoliths made from butyl methacrylate were fabricated in cyclic olefin copolymer microdevices and used for solid phase extraction. We studied the retention of fluorophores, amino acids and proteins on these columns. The retained samples were subsequently labeled with both Alexa Fluor 488 and Chromeo P503, and unreacted dye was rinsed off the column before sample elution. Additional purification was obtained from the differential retention of proteins and fluorescent labels. A linear relation between the eluted peak

SUBMITTER: Nge PN 

PROVIDER: S-EPMC3463737 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature