R-loops cause replication impairment and genome instability during meiosis.

ABSTRACT: R-loops are harmful structures with a negative impact on transcription and recombination during mitosis, but no information exists for meiosis. We used Saccharomyces cerevisiae and Caenorhabditis elegans THO mutants as a tool to determine the consequences of R-loops in meiosis. We found that both S. cerevisiae and C. elegans THO mutants show defective meiosis and an impairment of premeiotic replication as well as DNA-damage accumulation. Importantly, RNase H partially suppressed the replication impairment and the DNA-damage accumulation. We conclude that R-loops can form during meiosis causing replication impairment with deleterious results.

SUBMITTER: Castellano-Pozo M 

PROVIDER: S-EPMC3463965 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature