Solution structure, copper binding and backbone dynamics of recombinant Ber e 1-the major allergen from Brazil nut.

ABSTRACT:

Background

The 2S albumin Ber e 1 is the major allergen in Brazil nuts. Previous findings indicated that the protein alone does not cause an allergenic response in mice, but the addition of components from a Brazil nut lipid fraction were required. Structural details of Ber e 1 may contribute to the understanding of the allergenic properties of the protein and its potential interaction partners.

Methodology/principal findings

The solution structure of recombinant Ber e 1 was solved using NMR spectroscopy and measurements of the protein back bone dynamics at a residue-specific level were extracted using (15)N-spin relaxation. A hydrophobic cavity was identified in the structure of Ber e 1. Using the paramagnetic relaxation enhancement property of Cu(2+) in conjunction with NM

SUBMITTER: Rundqvist L 

PROVIDER: S-EPMC3464261 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature