Gene-based single nucleotide polymorphism markers for genetic and association mapping in common bean.

ABSTRACT:

Background

In common bean, expressed sequence tags (ESTs) are an underestimated source of gene-based markers such as insertion-deletions (Indels) or single-nucleotide polymorphisms (SNPs). However, due to the nature of these conserved sequences, detection of markers is difficult and portrays low levels of polymorphism. Therefore, development of intron-spanning EST-SNP markers can be a valuable resource for genetic experiments such as genetic mapping and association studies.

Results

In this study, a total of 313 new gene-based markers were developed at target genes. Intronic variation was deeply explored in order to capture more polymorphism. Introns were putatively identified after comparing the common bean ESTs with the soybean genome, and the primers were designed over int

SUBMITTER: Galeano CH 

PROVIDER: S-EPMC3464600 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature