Rapid genome-scale mapping of chromatin accessibility in tissue.

ABSTRACT:

Background

The challenge in extracting genome-wide chromatin features from limiting clinical samples poses a significant hurdle in identification of regulatory marks that impact the physiological or pathological state. Current methods that identify nuclease accessible chromatin are reliant on large amounts of purified nuclei as starting material. This complicates analysis of trace clinical tissue samples that are often stored frozen. We have developed an alternative nuclease based procedure to bypass nuclear preparation to interrogate nuclease accessible regions in frozen tissue samples.

Results

Here we introduce a novel technique that specifically identifies Tissue Accessible Chromatin (TACh). The TACh method uses pulverized frozen tissue as starting material and employs on

SUBMITTER: Grontved L 

PROVIDER: S-EPMC3464876 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature