PlantID - DNA-based identification of multiple medicinal plants in complex mixtures.

ABSTRACT:

Background

An efficient method for the identification of medicinal plant products is now a priority as the global demand increases. This study aims to develop a DNA-based method for the identification and authentication of plant species that can be implemented in the industry to aid compliance with regulations, based upon the economically important Hypericum perforatum L. (St John's Wort or Guan ye Lian Qiao).

Methods

The ITS regions of several Hypericum species were analysed to identify the most divergent regions and PCR primers were designed to anneal specifically to these regions in the different Hypericum species. Candidate primers were selected such that the amplicon produced by each species-specific reaction differed in size. The use of fluorescently labelled primers e

SUBMITTER: Howard C 

PROVIDER: S-EPMC3464944 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature