Mislocalized opsin and cAMP signaling: a mechanism for sprouting by rod cells in retinal degeneration.

ABSTRACT:

Purpose

In human retinal degeneration, rod photoreceptors reactively sprout neurites. The mechanism is unknown in part because of the paucity of animal models displaying this feature of human pathology. We tested the role of cAMP and opsin in sprouting by tiger salamander rod cells, photoreceptors that can produce reactive growth.

Methods

In vitro systems of isolated photoreceptor cells and intact neural retina were used. cAMP signaling was manipulated with nucleotide analogues, enzyme stimulators, agonists for adenosine and dopamine receptors, and the opsin agonist, β-ionone. Levels of cAMP were determined by radioimmunoassay, and protein levels by Western blot and quantitative immunocytochemistry. Neuritic growth was assayed by image analysis and conventional and confocal

SUBMITTER: Wang J 

PROVIDER: S-EPMC3465016 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature