Citrullination of proteins: a common post-translational modification pathway induced by different nanoparticles in vitro and in vivo.

ABSTRACT:

Aim

Rapidly expanding manufacture and use of nanomaterials emphasize the requirements for thorough assessment of health outcomes associated with novel applications. Post-translational protein modifications catalyzed by Ca(2+)-dependent peptidylargininedeiminases have been shown to trigger immune responses including autoantibody generation, a hallmark of immune complexes deposition in rheumatoid arthritis. Therefore, the aim of the study was to assess if nanoparticles are able to promote protein citrullination.

Materials & methods

Human A549 and THP-1 cells were exposed to silicon dioxide, carbon black or single-walled carbon nanotubes. C57BL/6 mice were exposed to respirable single-walled carbon nanotubes. Protein citrullination, peptidylargininedeiminases activity and targe

SUBMITTER: Mohamed BM 

PROVIDER: S-EPMC3465773 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature