Intralineage directional Notch signaling regulates self-renewal and differentiation of asymmetrically dividing radial glia.

ABSTRACT: Asymmetric division of progenitor/stem cells generates both self-renewing and differentiating progeny and is fundamental to development and regeneration. How this process is regulated in the vertebrate brain remains incompletely understood. Here, we use time-lapse imaging to track radial glia progenitor behavior in the developing zebrafish brain. We find that asymmetric division invariably generates a basal self-renewing daughter and an apical differentiating sibling. Gene expression and genetic mosaic analysis further show that the apical daughter is the source of Notch ligand that is essential to maintain higher Notch activity in the basal daughter. Notably, establishment of this intralineage and directional Notch signaling requires the intrinsic polarity regulator Partitioning defective

SUBMITTER: Dong Z 

PROVIDER: S-EPMC3466114 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature