Inducible control of subcellular RNA localization using a synthetic protein-RNA aptamer interaction.

ABSTRACT: Evidence is accumulating in support of the functional importance of subcellular RNA localization in diverse biological contexts. In different cell types, distinct RNA localization patterns are frequently observed, and the available data indicate that this is achieved through a series of highly coordinated events. Classically, cis-elements within the RNA to be localized are recognized by RNA-binding proteins (RBPs), which then direct specific localization of a target RNA. Until now, the precise control of the spatiotemporal parameters inherent to regulating RNA localization has not been experimentally possible. Here, we demonstrate the development and use of a chemically-inducible RNA-protein interaction to regulate subcellular RNA localization. Our system is composed primarily of two parts

SUBMITTER: Belmont BJ 

PROVIDER: S-EPMC3466194 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature