Development and validation of a new in vitro assay designed to measure contact allergen-triggered oxidative stress in dendritic cells.

ABSTRACT:

Background

Selected contact allergens are known to induce phenotypic and functional maturation of dendritic cells (DCs). Such changes occurring in DCs have been employed as assay readouts to predict skin-sensitizing potentials of small chemicals.

Objective

To respond to the urgent needs for reliable in vitro tests to identify contact allergens, we sought to develop a DC-based assay designed to detect early change(s) induced by sensitizers.

Methods

Signature gene expression profiles of skin sensitization were determined by GeneChip and quantitative RT-PCR analyses of RNA samples harvested from mouse skin and XS106 DC line after exposure to dinitrofluorobenzene (DNFB). Production of reactive oxygen species (ROS) was examined indirectly by measuring the level of oxidativ

SUBMITTER: Miyazawa M 

PROVIDER: S-EPMC3466341 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature