Chemically-blocked antibody microarray for multiplexed high-throughput profiling of specific protein glycosylation in complex samples.

ABSTRACT: In this study, we describe an effective protocol for use in a multiplexed high-throughput antibody microarray with glycan binding protein detection that allows for the glycosylation profiling of specific proteins. Glycosylation of proteins is the most prevalent post-translational modification found on proteins, and leads diversified modifications of the physical, chemical, and biological properties of proteins. Because the glycosylation machinery is particularly susceptible to disease progression and malignant transformation, aberrant glycosylation has been recognized as early detection biomarkers for cancer and other diseases. However, current methods to study protein glycosylation typically are too complicated or expensive for use in most normal laboratory or clinical settings and a more

SUBMITTER: Lu C 

PROVIDER: S-EPMC3466934 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature