Previously transmitted HIV-1 strains are preferentially selected during subsequent sexual transmissions.

ABSTRACT:

Background

A genetic bottleneck is known to exist for human immunodeficiency virus (HIV) at the point of sexual transmission. However, the nature of this bottleneck and its effect on viral diversity over time is unclear.

Methods

Interhost and intrahost HIV diversity was analyzed in a stable population in Rakai, Uganda, from 1994 to 2002. HIV-1 envelope sequences from both individuals in initially HIV-discordant relationships in which transmission occurred later were examined using Sanger sequencing of bulk polymerase chain reaction (PCR) products (for 22 couples), clonal analysis (for 3), and next-generation deep sequencing (for 9).

Results

Intrahost viral diversity was significantly higher than changes in interhost diversity (P < .01). The majority of HIV-1-discordan

SUBMITTER: Redd AD 

PROVIDER: S-EPMC3466994 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature