Dissection of cortical microcircuits by single-neuron stimulation in vivo.

ABSTRACT:

Background

A fundamental process underlying all brain functions is the propagation of spiking activity in networks of excitatory and inhibitory neurons. In the neocortex, although functional connections between pairs of neurons have been studied extensively in brain slices, they remain poorly characterized in vivo, where the high background activity, global brain states, and neuromodulation can powerfully influence synaptic transmission. To understand how spikes are transmitted in cortical circuits in vivo, we used two-photon calcium imaging to monitor ensemble activity and targeted patching to stimulate a single neuron in mouse visual cortex.

Results

Burst spiking of a single pyramidal neuron can drive spiking activity in both excitatory and inhibitory neurons within a ∼100

SUBMITTER: Kwan AC 

PROVIDER: S-EPMC3467311 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature