Development of a porcine (Sus scofa) embryo-specific microarray: array annotation and validation.

ABSTRACT:

Background

The domestic pig is an important livestock species and there is strong interest in the factors that affect the development of viable embryos and offspring in this species. A limited understanding of the molecular mechanisms involved in early embryonic development has inhibited our ability to fully elucidate these factors. Next generation deep sequencing and microarray technologies are powerful tools for delineation of molecular pathways involved in the developing embryo.

Results

Here we present the development of a porcine-embryo-specific microarray platform created from a large expressed sequence tag (EST) analysis generated by Roche/454 next-generation sequencing of cDNAs constructed from critical stages of in vivo or in vitro porcine preimplantation embryos. Tw

SUBMITTER: Tsoi S 

PROVIDER: S-EPMC3468353 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature