Measuring the methylome in clinical samples: improved processing of the Infinium Human Methylation450 BeadChip Array.

ABSTRACT: The Infinium Human Methylation450 BeadChip Array (TM) (Infinium 450K) is an important tool for studying epigenetic patterns associated with disease. This array offers a high-throughput, low cost alternative to more comprehensive sequencing-based methodologies. Here we compare data generated by interrogation of the same seven clinical samples by Infinium 450K and reduced representation bisulfite sequencing (RRBS). This is the largest data set comparing Infinium 450K array to the comprehensive RRBS methodology reported so far. We show good agreement between the two methodologies. A read depth of four or more reads in the RRBS data was sufficient to achieve good agreement with Infinium 450K. However, we observe that intermediate methylation values (20-80%) are more variable between technolog

SUBMITTER: Pan H 

PROVIDER: S-EPMC3469459 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature