Developing neurons form transient nanotubes facilitating electrical coupling and calcium signaling with distant astrocytes.

ABSTRACT: Despite the well-documented cooperation between neurons and astrocytes little is known as to how these interactions are initiated. We show here by differential interference contrast microscopy that immature hippocampal neurons generated short protrusions towards astrocytes resulting in tunneling nanotube (TNT) formation with an average lifetime of 15 minutes. Fluorescence microscopy revealed that all TNTs between the two cell types contained microtubules but 35% of them were F-actin negative. Immunolabeling against connexin 43 showed that this gap junction marker localized at the contact site of TNTs with astrocytes. Using optical membrane-potential measurements combined with mechanical stimulation, we observed that ~35% of immature neurons were electrically coupled with distant astrocytes

SUBMITTER: Wang X 

PROVIDER: S-EPMC3469499 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature