Analysis of Litopenaeus vannamei transcriptome using the next-generation DNA sequencing technique.

ABSTRACT:

Background

Pacific white shrimp (Litopenaeus vannamei), the major species of farmed shrimps in the world, has been attracting extensive studies, which require more and more genome background knowledge. The now available transcriptome data of L. vannamei are insufficient for research requirements, and have not been adequately assembled and annotated.

Methodology/principal findings

This is the first study that used a next-generation high-throughput DNA sequencing technique, the Solexa/Illumina GA II method, to analyze the transcriptome from whole bodies of L. vannamei larvae. More than 2.4 Gb of raw data were generated, and 109,169 unigenes with a mean length of 396 bp were assembled using the SOAP denovo software. 73,505 unigenes (>200 bp) with good quality sequences were sel

SUBMITTER: Li C 

PROVIDER: S-EPMC3469548 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature