Combining laser microdissection and RNA-seq to chart the transcriptional landscape of fungal development.

ABSTRACT:

Background

During sexual development, filamentous ascomycetes form complex, three-dimensional fruiting bodies for the protection and dispersal of sexual spores. Fruiting bodies contain a number of cell types not found in vegetative mycelium, and these morphological differences are thought to be mediated by changes in gene expression. However, little is known about the spatial distribution of gene expression in fungal development. Here, we used laser microdissection (LM) and RNA-seq to determine gene expression patterns in young fruiting bodies (protoperithecia) and non-reproductive mycelia of the ascomycete Sordaria macrospora.

Results

Quantitative analysis showed major differences in the gene expression patterns between protoperithecia and total mycelium. Among the genes st

SUBMITTER: Teichert I 

PROVIDER: S-EPMC3472292 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature