Yellow lupin (Lupinus luteus L.) transcriptome sequencing: molecular marker development and comparative studies.

ABSTRACT:

Background

Yellow lupin (Lupinus luteus L.) is a minor legume crop characterized by its high seed protein content. Although grown in several temperate countries, its orphan condition has limited the generation of genomic tools to aid breeding efforts to improve yield and nutritional quality. In this study, we report the construction of 454-expresed sequence tag (EST) libraries, carried out comparative studies between L. luteus and model legume species, developed a comprehensive set of EST-simple sequence repeat (SSR) markers, and validated their utility on diversity studies and transferability to related species.

Results

Two runs of 454 pyrosequencing yielded 205 Mb and 530 Mb of sequence data for L1 (young leaves, buds and flowers) and L2 (immature seeds) EST- libraries. A

SUBMITTER: Parra-Gonzalez LB 

PROVIDER: S-EPMC3472298 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature