Global analysis of genome, transcriptome and proteome reveals the response to aneuploidy in human cells.

ABSTRACT: Extra chromosome copies markedly alter the physiology of eukaryotic cells, but the underlying reasons are not well understood. We created human trisomic and tetrasomic cell lines and determined the quantitative changes in their transcriptome and proteome in comparison with their diploid counterparts. We found that whereas transcription levels reflect the chromosome copy number changes, the abundance of some proteins, such as subunits of protein complexes and protein kinases, is reduced toward diploid levels. Furthermore, using the quantitative data we investigated the changes of cellular pathways in response to aneuploidy. This analysis revealed specific and uniform alterations in pathway regulation in cells with extra chromosomes. For example, the DNA and RNA metabolism pathways were down

SUBMITTER: Stingele S 

PROVIDER: S-EPMC3472693 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature