In depth comparison of an individual's DNA and its lymphoblastoid cell line using whole genome sequencing.

ABSTRACT:

Background

A detailed analysis of whole genomes can be now achieved with next generation sequencing. Epstein Barr Virus (EBV) transformation is a widely used strategy in clinical research to obtain an unlimited source of a subject's DNA. Although the mechanism of transformation and immortalization by EBV is relatively well known at the transcriptional and proteomic level, the genetic consequences of EBV transformation are less well understood. A detailed analysis of the genetic alterations introduced by EBV transformation is highly relevant, as it will inform on the usefulness and limitations of this approach.

Results

We used whole genome sequencing to assess the genomic signature of a low-passage lymphoblastoid cell line (LCL). Specifically, we sequenced the full genome (40

SUBMITTER: Nickles D 

PROVIDER: S-EPMC3473256 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature