Transcriptome sequencing for SNP discovery across Cucumis melo.

ABSTRACT:

Background

Melon (Cucumis melo L.) is a highly diverse species that is cultivated worldwide. Recent advances in massively parallel sequencing have begun to allow the study of nucleotide diversity in this species. The Sanger method combined with medium-throughput 454 technology were used in a previous study to analyze the genetic diversity of germplasm representing 3 botanical varieties, yielding a collection of about 40,000 SNPs distributed in 14,000 unigenes. However, the usefulness of this resource is limited as the sequenced genotypes do not represent the whole diversity of the species, which is divided into two subspecies with many botanical varieties variable in plant, flowering, and fruit traits, as well as in stress response. As a first step to extensively document levels an

SUBMITTER: Blanca J 

PROVIDER: S-EPMC3473316 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature