Mate pair sequencing of whole-genome-amplified DNA following laser capture microdissection of prostate cancer.

ABSTRACT: High-throughput next-generation sequencing provides a revolutionary platform to unravel the precise DNA aberrations concealed within subgroups of tumour cells. However, in many instances, the limited number of cells makes the application of this technology in tumour heterogeneity studies a challenge. In order to address these limitations, we present a novel methodology to partner laser capture microdissection (LCM) with sequencing platforms, through a whole-genome amplification (WGA) protocol performed in situ directly on LCM engrafted cells. We further adapted current Illumina mate pair (MP) sequencing protocols to the input of WGA DNA and used this technology to investigate large genomic rearrangements in adjacent Gleason Pattern 3 and 4 prostate tumours separately collected by LCM. Sequ

SUBMITTER: Murphy SJ 

PROVIDER: S-EPMC3473372 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature