Glutaredoxin 1 protects dopaminergic cells by increased protein glutathionylation in experimental Parkinson's disease.

ABSTRACT:

Aims

Chronic exposure to environmental toxicants, such as paraquat, has been suggested as a risk factor for Parkinson's disease (PD). Although dopaminergic cell death in PD is associated with oxidative damage, the molecular mechanisms involved remain elusive. Glutaredoxins (GRXs) utilize the reducing power of glutathione to modulate redox-dependent signaling pathways by protein glutathionylation. We aimed to determine the role of GRX1 and protein glutathionylation in dopaminergic cell death.

Results

In dopaminergic cells, toxicity induced by paraquat or 6-hydroxydopamine (6-OHDA) was inhibited by GRX1 overexpression, while its knock-down sensitized cells to paraquat-induced cell death. Dopaminergic cell death was paralleled by protein deglutathionylation, and this was revers

SUBMITTER: Rodriguez-Rocha H 

PROVIDER: S-EPMC3474191 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature