Single-cell metabolomics: changes in the metabolome of freshly isolated and cultured neurons.

ABSTRACT: Metabolites are involved in a diverse range of intracellular processes, including a cell's response to a changing extracellular environment. Using single-cell capillary electrophoresis coupled to electrospray ionization mass spectrometry, we investigated how placing individual identified neurons in culture affects their metabolic profile. First, glycerol-based cell stabilization was evaluated using metacerebral neurons from Aplysia californica; the measurement error was reduced from ∼24% relative standard deviation to ∼6% for glycerol-stabilized cells compared to those isolated without glycerol stabilization. In order to determine the changes induced by culturing, 14 freshly isolated and 11 overnight-cultured neurons of two metabolically distinct cell types from A. californica, the B1 and

SUBMITTER: Nemes P 

PROVIDER: S-EPMC3474288 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature