The efficacy of detecting variants with small effects on the Affymetrix 6.0 platform using pooled DNA.

ABSTRACT: Genome-wide genotyping of a cohort using pools rather than individual samples has long been proposed as a cost-saving alternative for performing genome-wide association (GWA) studies. However, successful disease gene mapping using pooled genotyping has thus far been limited to detecting common variants with large effect sizes, which tend not to exist for many complex common diseases or traits. Therefore, for DNA pooling to be a viable strategy for conducting GWA studies, it is important to determine whether commonly used genome-wide SNP array platforms such as the Affymetrix 6.0 array can reliably detect common variants of small effect sizes using pooled DNA. Taking obesity and age at menarche as examples of human complex traits, we assessed the feasibility of genome-wide genotyping of poo

SUBMITTER: Chiang CW 

PROVIDER: S-EPMC3474315 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature