Development of Global Consensus Sequence and Analysis of Highly Conserved Domains of the HCV NS5B Prote in.

ABSTRACT:

Background

Hepatitis C virus (HCV) is a plus stranded RNA virus which encodes 10 different genes. The HCV NS5B gene encodes a polymerase, which is responsible for the replication of the virus and is a potential target for the development of antiviral agents. HCV has a high mutation rate and is classified into six major genotypes.

Objectives

The aim of this study was to draw a representing consensus sequence of each HCV genotype, align all six consensus sequences to draw a global consensus sequence and also study the highly conserved residues.

Materials and methods

236 HCV NS5B sequences, belonging to all six genotypes, reported from all over the world were aligned then a representing phylogenetic tree wasdrawn.

Results

The active site residues D220, D225, D318

SUBMITTER: Waheed Y 

PROVIDER: S-EPMC3475062 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature