One third of middle ear effusions from children undergoing tympanostomy tube placement had multiple bacterial pathogens.

ABSTRACT:

Background

Because previous studies have indicated that otitis media may be a polymicrobial disease, we prospectively analyzed middle ear effusions of children undergoing tympanostomy tube placement with multiplex polymerase chain reaction for four otopathogens.

Methods

Middle ear effusions from 207 children undergoing routine tympanostomy tube placement were collected and were classified by the surgeon as acute otitis media (AOM) for purulent effusions and as otitis media with effusion (OME) for non-purulent effusions. DNA was isolated from these samples and analyzed with multiplex polymerase chain reaction for Haemophilus influenzae, Streptococcus pneumoniae, Alloiococcus otitidis, and Moraxella catarrhalis.

Results

119 (57%) of 207 patients were PCR positive for at

SUBMITTER: Holder RC 

PROVIDER: S-EPMC3475091 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature