Rational improvement of the engineered isobutanol-producing Bacillus subtilis by elementary mode analysis.

ABSTRACT:

Background

Isobutanol is considered as a leading candidate for the replacement of current fossil fuels, and expected to be produced biotechnologically. Owing to the valuable features, Bacillus subtilis has been engineered as an isobutanol producer, whereas it needs to be further optimized for more efficient production. Since elementary mode analysis (EMA) is a powerful tool for systematical analysis of metabolic network structures and cell metabolism, it might be of great importance in the rational strain improvement.

Results

Metabolic network of the isobutanol-producing B. subtilis BSUL03 was first constructed for EMA. Considering the actual cellular physiological state, 239 elementary modes (EMs) were screened from total 11,342 EMs for potential target prediction. On this

SUBMITTER: Li S 

PROVIDER: S-EPMC3475101 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature