Identification of rat Rosa26 locus enables generation of knock-in rat lines ubiquitously expressing tdTomato.

ABSTRACT: Recent discovery of a method for derivation and culture of germline-competent rat pluripotent stem cells (PSCs) enables generation of transgenic rats or knock-out rats via genetic modification of such PSCs. This opens the way to use rats, as is routine in mice, for analyses of gene functions or physiological features. In mouse or human, one widely used technique to express a gene of interest stably and ubiquitously is to insert that gene into the Rosa26 locus via gene targeting of PSCs. Rosa26 knock-in mice conditionally expressing a reporter or a toxin gene have contributed to tracing or ablation of specific cell lineages. We successfully identified a rat orthologue of the mouse Rosa26 locus. Insertion of tdTomato, a variant of red fluorescent protein, into the Rosa26 locus of PSCs of var

SUBMITTER: Kobayashi T 

PROVIDER: S-EPMC3475147 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature