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An engineered lantipeptide synthetase serves as a general leader peptide-dependent kinase.

ABSTRACT: Phosphorylation is an abundant post-translational modification involved in a myriad of cell signaling pathways. Herein, we have engineered the class II lantipeptide synthetase ProcM to generate a variety of peptides containing O-phosphoserine (pSer) and O-phosphothreonine (pThr) residues, either in vitro or in vivo.

SUBMITTER: Thibodeaux GN

PROVIDER: S-EPMC3475617 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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An engineered lantipeptide synthetase serves as a general leader peptide-dependent kinase.

Thibodeaux Gabrielle N GN van der Donk Wilfred A WA

Chemical communications (Cambridge, England) 20121101 86

Phosphorylation is an abundant post-translational modification involved in a myriad of cell signaling pathways. Herein, we have engineered the class II lantipeptide synthetase ProcM to generate a variety of peptides containing O-phosphoserine (pSer) and O-phosphothreonine (pThr) residues, either in vitro or in vivo. ...[more]

PMID: 23001385

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