A simple and efficient transient transformation for hybrid aspen (Populus tremula × P. tremuloides).

ABSTRACT:

Background

The genus Populus is accepted as a model system for molecular tree biology. To investigate gene functions in Populus spp. trees, generating stable transgenic lines is the common technique for functional genetic studies. However, a limited number of genes have been targeted due to the lengthy transgenic process. Transient transformation assays complementing stable transformation have significant advantages for rapid in vivo assessment of gene function. The aim of this study is to develop a simple and efficient transient transformation for hybrid aspen and to provide its potential applications for functional genomic approaches.

Results

We developed an in planta transient transformation assay for young hybrid aspen cuttings using Agrobacterium-mediated vacuum infiltr

SUBMITTER: Takata N 

PROVIDER: S-EPMC3476444 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature