Multifactorial patterns of gene expression in colonic epithelial cells predict disease phenotypes in experimental colitis.

ABSTRACT:

Background

The pathogenesis of inflammatory bowel disease (IBD) is complex and the need to identify molecular biomarkers is critical. Epithelial cells play a central role in maintaining intestinal homeostasis. We previously identified five "signature" biomarkers in colonic epithelial cells (CEC) that are predictive of disease phenotype in Crohn's disease. Here we investigate the ability of CEC biomarkers to define the mechanism and severity of intestinal inflammation.

Methods

We analyzed the expression of RelA, A20, pIgR, tumor necrosis factor (TNF), and macrophage inflammatory protein (MIP)-2 in CEC of mice with dextran sodium sulfate (DSS) acute colitis or T-cell-mediated chronic colitis. Factor analysis was used to combine the five biomarkers into two multifactorial princ

SUBMITTER: Frantz AL 

PROVIDER: S-EPMC3476470 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature