Computational identification of transcriptionally co-regulated genes, validation with the four ANT isoform genes.

ABSTRACT:

Background

The analysis of gene promoters is essential to understand the mechanisms of transcriptional regulation required under the effects of physiological processes, nutritional intake or pathologies. In higher eukaryotes, transcriptional regulation implies the recruitment of a set of regulatory proteins that bind on combinations of nucleotide motifs. We developed a computational analysis of promoter nucleotide sequences, to identify co-regulated genes by combining several programs that allowed us to build regulatory models and perform a crossed analysis on several databases. This strategy was tested on a set of four human genes encoding isoforms 1 to 4 of the mitochondrial ADP/ATP carrier ANT. Each isoform has a specific tissue expression profile linked to its role in cellular

SUBMITTER: Dupont PY 

PROVIDER: S-EPMC3477019 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature