Combinatorial assembly of developmental stage-specific enhancers controls gene expression programs during human erythropoiesis.

ABSTRACT: Gene-distal enhancers are critical for tissue-specific gene expression, but their genomic determinants within a specific lineage at different stages of development are unknown. Here we profile chromatin state maps, transcription factor occupancy, and gene expression profiles during human erythroid development at fetal and adult stages. Comparative analyses of human erythropoiesis identify developmental stage-specific enhancers as primary determinants of stage-specific gene expression programs. We find that erythroid master regulators GATA1 and TAL1 act cooperatively within active enhancers but confer little predictive value for stage specificity. Instead, a set of stage-specific coregulators collaborates with master regulators and contributes to differential gene expression. We further ide

SUBMITTER: Xu J 

PROVIDER: S-EPMC3477283 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature