Genomic profiling of a human leukemic monocytic cell-line (THP-1) exposed to alpha particle radiation.

ABSTRACT: This study examined alpha (α-) particle radiation effects on global changes in gene expression in human leukemic monocytic cells (THP-1) for the purposes of mining for candidate biomarkers that could be used for the development of a biological assessment tool. THP-1 cells were exposed to α-particle radiation at a dose range of 0 to 1.5 Gy. Twenty-four hours and three days after exposure gene expression was monitored using microarray technology. A total of 16 genes were dose responsive and classified as early onset due to their expression 24 h after exposure. Forty-eight transcripts were dose responsive and classified as late-onset as they were expressed 72 h after exposure. Among these genes, 6 genes were time and dose responsive and validated further using alternate technology. These tran

SUBMITTER: Chauhan V 

PROVIDER: S-EPMC3477555 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature