At3g08030 transcript: a molecular marker of seed ageing.

ABSTRACT:

Background and aims

Prolonged storage generally reduces seed viability and vigour, although the rate of deterioration varies among species and environmental conditions. Here, we suggest a possible ageing molecular marker: At3g08030 mRNA. At3g08030 is a member of the DUF642 highly conserved family of cell-wall-associated proteins that is specific for spermatophytes.

Methods

At3g08030 expression was performed by RT-PCR and qRT-PCR analysis in seed samples differing in their rate of germination and final germination following a matrix priming and/or controlled deterioration (rapid ageing) treatment.

Key results

The At3g08030 gene transcript was present during the entire Arabidopsis thaliana plant life cycle and in seeds, during maturation, the ripening period and after g

SUBMITTER: Garza-Caligaris LE 

PROVIDER: S-EPMC3478058 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature